Effects of H2O2 conc with catalyst ---- michaelis equation demonstration
I have come across a major stumbling block with my kinetics (michaelis equation).
How do I predict using the michaelis equation the volume of oxygen collected for the given concentrations? Please could you give an example of the complete working out for any given concentration, where necessary (if required) making up values of any data I omitted. ----- see below -----
Aim:
I am investigating the changes of hydrogen peroxide concentration on the rate of decomposition by catalase.
Outline of method:
The apparatus involve yeast(catalase), hydrogen peroxide, a 100cm3 gas syringe to collect oxygen. I am calculating the volume of gas collected in 30 seconds. The concentrations I plan to do are 0.1M, 0.5M, 1M, 2M, 4M. The concentration of the yeast is 10g of bakers yeast granules dissolved in 100g of water.
(I am interested in the demonstrated use of the equation not general information concerning the topic)
By OTA: Qiguang Li, PhD (IP)
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