Genetics

Consider the following artificially created yeast Ty element: See attached file for full problem description. Upstream from the element has been placed the very strong PGAL promoter which is induced by galactose added to the media. The transcript traverses the whole element (large dashed arrow). Downstream from the normal Ty Open Reading Frames was inserted the yeast HIS3 gene, including its own promoter (small dashed arrow shows transcript), but in reverse orientation. Inserted into the middle of HIS3, and therefore blocking its expression, is an Intervening Sequence (IVS) from another gene. The IVS orientation corresponds to the P GAL transcript (left-to-right). This construct w... click for more

Reconstructing a Tn10 phage element

When phage λ is grown on a Tn10-containing E. coli host, rare phage that transduce tetracycline-resistance can be isolated. You isolate DNA from λ and from λ-tet and cut each with EcoRI and BamHI and see the following sized fragments on an agarose gel: Tn10 is 9kb long, with one EcoRI site 3.5kb from its left end (and there are no BamHI sites). Construct a restriction map of λ showing the positions of the EcoRI and BamHI sites and the position and orientation of the Tn10 insertion in λ-tet. See attached file for full problem description.

Biology/Genetics Questions

Biology/Genetics Questions. See attached file for full problem description.

Genetic Biology Questions

Genetic Biology Questions. See attached file for full problem description.

Human chromosomes and formulas; abnormal chromosome number

I am having trouble writing the formulas. I know what each X and Y chromosome each possess but I don't know who to write a formula with that. 1. Write a formula for: (a) a male with Down syndrome (due to the presence of an extra chromosome 21) (b) a female with Turner syndrome (only one X chromosome) (c) a female with cri du chat syndrome (deletion of a portion of p arm of chromosome 5) (d) a male with Klinefelter syndrome (two X chromosomes and one Y chromosome) (e) a female with a Philadelphia chromosome (tanslocation between chromosomes 9 and 22). People suffering from a form of leukemia (chronic myeloid leukemia) frequently harbor cells containing the Philadelphia chromosome. ... click for more