Inverted and direct repeat

Imagine that you find a sequence 5' GTTTCA 3' at one end of a transposable element. a) Draw this sequence as an inverted repeat on both ends of the element (filling in the N's in the following format): 5GTTTCA-------------------------------NNNNNN3 3CAAAGT------------------------------NNNNNN5 B) draw this sequence as a direct repeat using the same format as above.

Nuclear transplantation

Several species of mammals have been cloned by nuclear transplantation using an adult cell as the source of the nucleus. Is there any contribution to the genotype of the cloned offspring from the oocyte into which the nucleus is tranplanted? Briefly explain. Name a technique that can be used to simulataneously detect the transcripts of thousands of genes in a single tissue sample.

Probability

Answer the following questions based on the assumption that genotypes BB and Bb have brown eyes, while bb has blue eyes. Filled in the symbols in the pedigrees that represent blue eyes. a) What is the probability that the first child of a mating between II-2 and II-7 would be a blue-eyed girl? b) What is the probability that the first child of a mating between II-3 and II-8 would be blue-eyed? c) What is the probability that II-6 is homozygous? d) What is the probability that the first child of II-3 and II-8 would be a brown-eyed boy? (see diagram in the attached file)

Probability

(see diagram in the attached file) Answer the following questions based on the assumption that genotypes BB and Bb have brown eyes, while bb has blue eyes. Filled in the symbols in the pedigrees that represent blue eyes. a) What is the probability that the first child of a mating between II-2 and II-7 would be a blue-eyed girl? b) What is the probability that the first child of a mating between II-3 and II-8 would be blue-eyed? c) What is the probability that II-6 is homozygous? d) What is the probability that the first child of II-3 and II-8 would be a brown-eyed boy?

Electrophoresis

Regarding exercise 12(see attachment)………These are the measurements from electrophoresis well 1=37mm,36,29,25,22,18 and well 2-5 all have measurements of 45mm can you do this equation for me as stated on page 105-106 dealing with the regression line equation. Why are different temperatures needed for the 3 steps in the polymerase chain reaction?