Drosophila linkage relationship

An experiment was done to determine the linkage relationship of three genes (a,b,c) in drosophila melanogaster. Homozygous females phenotypically a,c were crossed with homozygous males phenotypically b. The F1 females were all wild-type in apearance and the F1 males were all a,c. The F females and males were crossed to give the following F2 phenotypes and numbers: Males Females a+c 242 310 +b+ 238 0 ab+ 157 0 ++c 163 190 abc 59 0 +++ 61 295 a++ 38 205 +bc 42 0 Total 1000 1000 a) give the genotypes of the parents b) give the genotypes of the... click for more

Restriction sites

In a restriction mapping experiment using the plasmid P (ST), you cleave the circular plasmid with the restriction enzymes Xbal, Asp718, BamHI and EcoRI individually and in various combinations with each other. You obtain DNA fragments with the following sizes: Enzymes used size of DNA fragments (bp) BamHI, EcoRI, Xbal 3000, 1550 Asp718, BamHI, Xbal 3000, 2500, 600 Asp718, BamHI 5500, 600 Asp718, Xbal 3600, 2500 BamHI, Xbal 3100, 3000 Asp718 6100 BamHI 6100 Xbal 6100 Using the data above, make a map of the P (ST) plasmi... click for more

Chi squared analysis

In corn, colored aleurone (R) is dominant to colorless aleurone (r), and green plant color (G) is dominant to yellow plant color (g). two plants, each heterozygous for both characteristics, are testcrossed to homozygous recessives, and their progeny are combined to produce the following totals: colored green 100 colored yellow 97 colorless green 103 colorless yellow 100 a) use chi-squared analysis to test these data for independent assortment of the two characteristics. b) when the progeny of each of the two heterozygous plants are scored separately, the following results are obtained: phenotypes plant 1 plant 2 colored green 88 12... click for more

DNA Questions

A. Biotechnology cannot be used to: ___________ a. Produce large quantities of particular human proteins. b. Produce effective and safe vaccines. c. Identify human fetuses with particular genetic diseases. d. Alter food plants to increase yield. e. Alter the intelligence levels of newborn infants. B. For these questions below, use the following list. Each selection may be used more than once and each statement may have more than one correct answer. a. Herbicide resistant corn b. Bt toxin producing soybean c. Banana vaccine against pathogenic E. coli d. Growth hormone treated cows e. Human insulin produced by bacteria f. Human Gene Therapy 1. Example(s) o... click for more

Determining the mode of inheritance and gene linkage for drosophila crosses...

7. Conclusion and discussion sections: a. how many genes are involved in creating the eye pigment (based on the provided data). Mode of inheritance - are the genes autosomal or sex-linked and why, recessive or dominant and why, and are some genes linked and why. b. Hypothesis statement based on conclusions about linkage from previous step (7a), and chi square test to accept or reject the hypothesis. c. You should include punnett squares with genotypes to all generations of all 4 crosses! I have connected the data table that we were given, and I have also given my explanation for crosses 1 and 3. However, I have no idea how 2 and 4 are linked, i have never seen a 14... click for more