Analysis of a Cell-Surface Receptor

Questions: 1. For Part A, please explain in detail the results in Data Analysis and why they turned out as they did? 2. For Part B, please explain in detail the results in Data Analysis and why they turned out as they did? 3. One of the simple sugars (galactose or mannose) used in this exercise should have inhibited Con A-peroxidase binding to cheek epithelial cells and the Con A-induced hemoagglutination reaction. Name this sugar and describe the mechanism by which it inhibits these effects? 4. Describe the mechanism responsible for the Con-A induced hemoagglutinating reaction? 5. Most membrane proteins including the Con-A receptor can be solubilized by treating cell membranes ... click for more

ConA Receptor

Most membrane proteins including the Con-A receptor can be solubilized by treating cell membranes with a non-ionic detergent such as Triton X 100. You are given such a soluble preparation of erythrocyte proteins. Describe a single procedure that could be used to isolate the Con A Receptor from this preparation? Please explain.

Images of phyla members

Questions on images of phyla members. See attached file for full problem description.

Cell Bio

1. Explain why the coupling of the import of amino acids or sugars into cells is typically linked to Na+ ion import. 2. What happens to the post-synaptic cells’ membrane potential at an inhibitory synapse? 3. Explain what pumps and/or transporters are involved in moving neurotransmitters into synaptic vesicles.

Cell Biology

1. An integrin is an integral membrane protein in the plasma membrane of cells. It plays a role in the attachment of a cell to the extracellular matrix (ECM) and to other cells, and in signal transduction from the ECM to the cell. How is the structure of integrins reflective of this function? 5. In a cell with a resting membrane potential what is the distribution of K+, Na+, and Ca2+ ions (more extracellulary or intracellurally). Explain hide problem