Concentration/Dilutions

There is 100 mg of protein dissolved in 100 mL water. Then 10 microliters of that solution is dissolved in 990 microliters of water for total volume of 1.0 mL. To calculate the concentration of protein is it 10/990 or 10/1000? And does the original concentration of solution even matter when calculating the solution concentration?

Basic Techniques in Cell Biology and Biochemistry

The only problems that need to be done are numbers (3) and (4) on the second scanned document and the question below . Numbers (1), (2), (5) are finished problems that do not need to be done so problems (3) and (4) should be done using the same format (do Calculations and preparation)as those. Also please answer this question: Prepare bovine serum albumin solutions (.2, .4, .6, and .8) from 1.0 mg/ml stock. Show how to do this in the same format and (1) and (2) with calculations and preparation.

Gene Expression

I do not understand the process of gene expression as given on certain cites. Please explain the process of gene expression in prokaryotes.

Eukaryotic Cell

1. Describe the changes in clarity and viscosity of a nuclear suspension that occurs upon the addition of Sodium Dodecyl Sulfate (SDS)? Why do they changes occur? 2. Thymus cells have a relatively small amount of cytoplasm and this feature makes thymus tissue ideal for DNA extraction. Why? 3. Describe the basic cellular events that occur in a onion root zone with the highest peroxidase activity?

Topic 5 Diffusion, Osmosis, and Biological Membranes (questions from the book- General Biology Laboratory Manual Investigations Into Life's Phenomena

Lab5A Procedure The molecules of all matter, particularly gases and liquids, are in constant vibration motion. As a result of this motion, molecules are continually colliding with one another, much like balls scattering over a pool table. Using a microscope, you can observe these molecular collisions that produce the effects of diffusion and osmosis 1.Obtain a slide and place two drops of water on it. set the slide down on the table. 2.stick a toothpick vertically down into the container of carmine due powder to a depth of about one half inch. Hold the toothpick vertically over the water drop on your slide with point just above the water. Gently tap the toothpick to shake a light sprinkl... click for more