Cell Biology Homework Questions

1. Bacteriophage M13 infects E. coli differently from the way bacteriophage T2 does. The M13 coat is removed in the inner membrane of the bacterial cell, where it is sequestered during phage replication. It is subsequently used to package the newly replicated phage DNA to create progeny phage. Why would this make M13 less suitable than T2 for the Hershey and Chase experiment? 2. Adenovirus has a double-stranded linear DNA genome that measures 12.2 m in length. How many base pairs make up the adenovirus genome? 3. A DNA is found to exhibit a melting point of 75C in 0.1 M NaCl. What effect would each of the following have on the malting point of the DNA? a.... click for more

Cell Biology Homework

1. When DNA samples A and B were separated by buoyant density centrifugation the two samples migrated 23 and 15 cm, respectively. If DNA B had a GC content of 34%, what was the GC content of DNA A? 2. If Meselson and Stahl found that the ratio of 15N-labeled single-strands to 14N-labeled single-strands was 2:6 in the second generation, what would be the ratios for the fourth and fifth generations? 3. In the first generation of growth on 15N containing media, a single band of DNA having an intermediate density was detected by buoyant density centrifugation using a neutral CsCl gradient. If the dispersive model of replication was true, what result would have been seen? What would b... click for more

Cell Biology Homework

1. Digestion of the oligonucleotide, AGUACAA, by pancreatic RNase would yield what products? 2. A 5.5 kb, circular plasmid is cut with the following restriction endonucleases, producing the fragments listed. Construct a restriction map for this plasmid. Endonuclease DNA fragments (kb) Eco RI 5.5 Pst I 5.5 Bam HI 2.5 and 3.0 Eco RI and Bam HI 1.5 and 2.5 Eco RI and Pst I 0.5 and 5.0 Bam HI and Pst I 1.0, 2.0 and 2.5

part of the lab final

i cant figure out what i am suppose to do for this assignement that we are to do. it says to apply each lab that we have done in the class to our career . for example the lab on osmossis and diffusion how would i make three applications for that lab?

The similarities and differences between prokaryotic and eukaryotic cells.

What are the similarities and differences between prokaryotic and eukaryotic cells?