Radioactive Ribosmal Subunits

You prepare radioactivley labelled ribosomal subunits and you microinject them into cells that you are culturing in the laboratory. At different times after you inject the subunits, you take a small amount of cells and analyze them by electron microscopy. You find very soon after injection, the labelled subunits are assembled into cytosolic ribosomes. As more time passes, labelled subunits can be seen in both cytosolic and ER-bound ribosomes. Interpret these observations.

Phospholipid bilayer

What are the three chief components of eukaryotic cell membranes? Where does each originate? What organelle synthesizes new membrane? What accounts for the stability of phospholipid bilayers in acqueos systems? Would the three components be the integral membrane protein, cholesterol, and lipid? Is the ER the organelle that synthesizes new membrane?

transport vesicles, secretory vesicles, and endocytotic vesicles

Whats the difference between transport vesicles, secretory vesicles, and endocytotic vesicles? I know that Transport vesicles move molecules between locations inside the cell. For example they move proteins from the ER to the Golgi, from there to the outer membrane, where they are secreted

Isolate peroxisomes

Hi, I have a test next wednesday and a concept is not clear to me. How would I isolate peroxisomes from cells. ( I dont need a complicated technical answer, but more of a general idea). Thanks

Cell Culture: The effect of short term variation in temperature and pH on the viability of mouse 3T3 cells

I was absent during the practical session which took place and am having great difficulty in attempting this problem. Basically, this is what is REQUIRED: 1. Calculate the total number of Viable and non-viable cells present in each cell suspension using the formula: Number of cells in 1ml of Trypan Blue: cell suspension = N/9 x 10(to the power 4) 2. Because the cell suspension was diluted by half with the trypan blue it is necessary to multiply by 2 to obtain the total number of cells in your original cell suspension. Discussion: Discuss the significance of your results I have attached the results in the file