COPII assembly

I am currently studying the intracellular distribution of a particular protein. The protein is normally Golgi-resident. What would happen to this distribution if i treated the cell with an inhibitor of COPII assembly? I would think that if the COPII vesicles are inhibited the ER would be unable to export cargo to the golgi.

Label Ribosomal Subunits

You prepare radioactivley labelled ribosomal subunits and you microinject them into cells that you are culturing in the laboratory. At different times after you inject the subunits, you take a small amount of cells and analyze them by electron microscopy. You find very soon after injection, the labelled subunits are assembled into cytosolic ribosomes. As more time passes, labelled subunits can be seen in both cytosolic and ER-bound ribosomes. You repeat the experiment except that at the same time you inject ribosomal subunits into the cells, you also inject the inhibitor of the ER translocon. Where will you find the labelled ribosomal subunits in the cell, both immediatley after the inje... click for more

ribosomal synthesis

A protein is synthesized on a ribosome. What are the different destinations where it might end up, and which require signal sequences.

Coat Proteins

The image shows the inner face of a cell membrane with numerous budding structures, each one surrounded by coat proteins. What process is going on, and what is the role of the coat proteins?

Permeability

What types of chemical can generally pass through phospholipid portions of the cell membrane and why? What types are generally prevented from passing through and why? I would guess hydrophillic chemicals can pass through.