Understanding the structure and stability of base pairing in DNA. Predicting relative melting temperature (Tm) for DNA fragments.

Two duplex (ie. double stranded) DNA fragments are shown below. Under identical conditions (ie. ionic strength, buffer composition, temperature, etc.), which double-stranded fragment would denature at the lower temperature? Fragment A: 5' A-A-G-T-A-G-T-T-T-C-A 3' 3' T-T-C-A-T-C-A-A-A-G-T 5' Fragment B: 5' G-C-A-G-G-T-C-G-C-C-T 3' 3' C-G-T-C-C-A-G-C-G-G-A 5'

Use your understanding of the regulation of enzymes within metabolic pathways to predict which steps of a metabolic pathway would be inhibited by the accumulation of various reaction products

A metabolic pathway is represented below. Each step is catalyzed by a different enzyme. (please refer to the attached file to see the reactions within the pathway) (a) Which step would be inhibited by the accumulation of the product E? (b) Which step would be inhibited by the accumulation of the product H? (c) By which mechanism of inhibition would you expect E and H to act?

Classifying different molecules as carbohydrates, lipids, nucleic acids or proteins.

Identify each of the following substances as a carbohydrate, lipid, nucleic acid, protein, or "none of these". (a) antibody (b) actin (c) fructose (d) cellulose (e) RNA (f) beta-galactosidase (g) heme (h) phospholipase A2 (i) glucose (j) tryptophan (k) glycogen (l) cholesterol (m) hemoglobin

Metabolism

How do you calculate specific activity, total activity, and % radioactivity given the information in the attachment?

An overview of glycolysis, the TCA cycle, and the electron transport chain and where each pathway occurs.

Describe the biochemical pathways in the aerobic respiration of glucose and where each takes place within the cell.