Genetic crosses with sex linked traits, various allels, mutations and a metabolic pathway. Attachments in MS Word.

In J.R.R Tolkien's The Lord of the Rings, the Black Riders of Mordor ride steeds with eyes of fire. As a geneticist, you are very interested in the inheritance of the fire-red eye colour. A discovery is made that the eyes contains two types of pigments, brown and red, that are usually bound to core granules in the eye. In wild-type steeds, precursors are converted by these granules to the above pigments, but in steeds homozygous for the recessive X-linked gene w (white eye), the granules remain unconverted and a white eye results. The metabolic pathways for the synthesis of the two pigments are shown in the attached file. Each step of the pathway is controlled by a gene: mutation v results ... click for more

Drawing a linear map representing the order and overlap of the deletions and the positions of the point mutations. Attachments in Word.

Five single rII deletion mutants of phage T4 were spot-tested in all pairwise combinations to test for their ability to give r+ recombinants. The results are given in Table (a). Five different point mutations were then tested in the same way for their ability to recombine with the five deletions. These results are given in Table (b). In both tables, "0" indicates no recombination, while "+" indicates that recombinants were detected. Using the information given, draw a linear map representing the order and overlap of the deletions and the positions of the point mutations. View the attachment below for the full question.

Determining haplotypes, recombination frequencies, and LOD scores. Attachments in MS Word.

An autosomal dominant disease (Huntington disease) is being transmitted in the pedigree below. A two-allele marker (designated 1 and 2) has been typed for each family member. What are the mother's haplotypes i.e. which alleles are inherited together? What is the recombination frequency for the marker and the disease locus? What is the LOD score for a recombination frequency of 0.0? What is the LOD score for a recombination frequency of 0.1? (Just use the progeny from generation III) View attachment below for pedigree.

Genetics

(a) Give the genotypes of the two parental goats, showing gene arrangement and gene order. (b) Give the % recombination between each pair of genes. (c) Draw a map showing the positions of all genes and distances between genes, where appropriate (d) Give the formula and calculate the coefficient of coincidence (e) Calculate interference. 2. Given the map a 10 b 20 d , and with no chromosome interference, how may phenotypically a+b+d individuals would you expect in a progeny of 1000 of the following cross: a+ b d+ / a b+ d X a b d / a b d ? See attached file for full problem description.

GENETICS

4. In yeast, wild type colonies are white. Adenine-requiring auxotrophs (ad) are red when grown on medium containing limiting amounts of adenine. This is due to the accumulation of a red precursor in adenine biosynthesis. If cells from an ad strain are plated on minimal medium most cells don’t produce colonies but two rare types of “revertant” colonies are produced, one type white in colour, and the other type with a “rose” colour. Strains grown from such colonies were crossed to wild type of opposite mating type an yielded the following results: white “revertant” X wild type (also white) Æ all white auxotrophic progeny rose “revertant” X wild type (white) Æ ½ white, ¼ rose, ¼ red (... click for more