Fatty Acids: Reactions, Mechanisms and Enzymes.

Purified fatty acid synthetase from liver cytoplasm normally catalyzes the sythesis of palmitate(C16) from acetyl CoA, malonyl CoA, and NADPH. The enzyme also will function, although at considerably reduced rates, if the acetyl CoA is replaced by (1) propionyl CoA or (2) butyryl CoA. a). What do you predict will be the major product of the reaction in each of the latter two cases? b). How many can the predictions above be used to determine the mechanism by which the fatty acids are released from the fatty acid synthetase?

Enzymes

The following experiments are carried out using a pure preparation of the enzyme that catalyzes palmitate synthesis from acetyl CoA and malonyl CoA in the presence of all the cofactors required for reaction. a). If the acetyl CoA is labeled at the methyl carbon with tritium, and malonyl CoA is unlabeled, how many tritium atoms will be incorporated into each molecule of palmitate formed? b). If malonyl CoA is supplied in the form of -OOC^3H2COSCoA, and acetyl CoA is unlabeled, how many tritium atoms will be incorporated into each molecule of palmitate formed?

GRE Biochem subject exam

Calculate the free energy change for translocating a proton out of the mitochondrial matrix, where pH(matrix) = 7.6 pH(cytosol) = 7.2 ΔΨ=200mV and T= 37.0C Please Explain. .. .

GRE Biochem subject exam

Consider the reaction catalyzed by alcohol dehydrogenase. (Reaction goes from left to right) CH3CH2OH + NAD+ <-----------> CH3COH + NADH +H+ WHAT IS THE OXIDIZING REAGENT? WHAT IS THE REDUCING AGENT? Please explain

GRE Biochem subject exam

What is ΔE for the oxidation of ubiquinol by cytochrome c when the ratio of [QH2]/[Q] is 10 and the ratio of [cyt c3+]/[cyt c2+] is 5? cytochrome c (Fe3+) + e- <---------> cytochrome c (Fe2+) E'˚ = 0.254V Ubiquinone + 2H+ + 2e- <-----------> ubiquinol + H2 E'˚ = 0.045V