Ionization State; Cooperative Ligand Binding

1. The active site of lysozyme contains two amino acid residues essential for catalysis: Glu35 and Asp52. The pka values of the carboxyl side chains of these low residues are 5.9 and 4.5 respectively. a) what is the ionization state(protonated or deprotonated) of each residue at pH5.2, the pH pH optimum of lysozyme? b) how can the ionization states of these residues explain the pH-activity profile of lysozyme? 2. which set of binding data is likely to represent cooperative ligand binding in an oligomeric protein? Explain. A.[ligand] Y(fractional saturation) B.[ligand] Y(fractional saturation) (mM) mM 0.1 ... click for more

Isomerization Reaction - Ratio; Steady State Concentrations; Cellular Conditions

Consider the isomerization reaction between glucose-6-phosphate and fructose-6-phosphate. A) What is the ratio of fructose-6-phosphate to glucose-6-phosphate under standard conditions? B) Under typical cellular conditions, the steady state concentrations of glucose-6-phosphate and fructose-6-phosphate are 0.083mM and 0.014mM. What is delta G' under cellular conditions? C) In what direction does the reaction proceed under cellular conditions? Please EXPLAIN

Reactions - Inhibit; Catalyse; Glycolosis

Why is it advantageous for citrate, the product of reaction 1 of the TCA, to inhibit phosphofructokinase, which catalyses the third reaction in glycolysis? PLEASE EXPLAIN

Reaction; Control Point; Citric Acid Cycle

The Δ G'˚ for the fumerase reaction is -3.4kJ/mol, but Δ G' is near 0. What is the ratio of fumarate to malate under cellular conditions at 37.0C? Is this reaction likely to be a control point in the citric acid cycle? Please Explain

Glucose as Intravenous Food Source

Glucose is frequently administered intravenonusly to patients as a food source. A new resident at the hospital suggests administering glucose-6-phosphate instead. Glucose-6-phosphate requires ATP and you consider the possibility that administering glucose-6-phosphate might save the patient energy. Should you use the residents suggestion? PLEASE Explain