GRE (Biochem) Subject Question

When quantifying the activity of an enzyme, does it matter wheter you measure the appearence of a product or the dissapearence of a reactant? Please explain this to me. . . .

GRE Biochem Subject

It is possible to distinguish between irreversible inhibitor and a classical mixed type reversible inhibitor by measuring Vmax of the enzymatic reaction as a function of [E]total in the absence and presence of the inhibitor. What does the slope represent in each case? What does the X-axis intercept represent in each case? PLEASE EXPLAIN. . . . I am missing something

GRE subject Biochem

How many different possible disaccharides can be formed between the pyranose forms od D-galactose and D-glucose? How many are nonreducing? Please explain or show the reasoning on this question .. .. .

GRE subject Biochem

Discuss the feasibility of using methylation analysis to distinguish between each of the following pairs of carbohydrates: A) Maltose and Trehalose B) Maltose and Amylose C) Amylose and Cellulose Please explain this . . .

Changes in pyridoxal phosphate structure on catalysis

2. Pyridoxal phosphate is required for transamination reactions. The mechanism of catalysis by pyridoxal phosphate can be studied free of enzyme, since pyridoxal itself (shown in attachment) is active towards substrates in the presence of a metal ion (the rate is significantly reduced, but can be measured). Consequently, one can study changes in pyridoxal phosphate structure on catalysis. Based on your understanding of the mechanism of action by pyridoxal phosphate, which of the analogues below would you expect to be active, and which would be inactive? Explain your answer. see attachment for diagram