Catalytic Function - Optimum pH

The catalytic function of most enzymes is a sensitive function of pH. The attached plot (activity vs pH) of the data will clearly illustrate the degree of this sensitivity. What is the optimum pH for this enzyme?

Calculate the Vmax and Km:

Please see attached problem. Thank you

Enzyme Kinetics: Hydrolysis-Rate Data (Find Km and Vmax)

An ATPase from yak saliva was isolated, and the following set of hydrolysis-rate data was obtained {view attachment}. Find Km and Vmax ...

Enzyme Kinetics: Lineweaver - Burke Plot

The attacheed data were obtained. Make a Lineweaver - Burke plot, showing how each inhibitor, l and K behaves differently from the uninhibited enzyme mixture and determine the nature of each inhibitor.

Michaelis-Menten Method; Effect of pH on Enzyme

Phosphofructokinase is an enzyme, widespread in nature, catalyzing the reaction: fructose-6-phosphate + ATP ----------> fructose-1,6-diphospohate + ADP Under conditions where the concentration of ATP was held constant, the dependency of the initial velocity of the concentration of fructose-6-phosphate as measured at two different pH values (6.9 and 8.2) is represented by the data attached. Plot the data according to the classical Michaelis-Menten method and interpret what effect the pH has on the activity of the enzyme.