Comparing the best inhibitor given km, kcat and ki

Here are the following numbers I have calculated; We were given a substrate to plot againist velocity and 3 enzymes to plot as well. From the data: Enzyme A Ki= 2.575 Kcat = 50 Km= 3.33 Enzyme B Ki = 10.33 Kcat = 16.65 Km = 33.3 Enzyme C Ki= 1.00 Kcat = 10 Km = .2 I'm having trouble understanding which is the best inhibitor b/c: A has the highest Kcat- best collision efficiency B has the highest Km - the highest affinity C has the lowest Ki- which means it binds the tightest. Which one is the best? Thank you, Rebecca

Solving Buffer Solution Problems

Calculate the concentration of acetic acid and sodium acetate necessary to prepare a buffer solution of pH = 5.1 that is 0.25M in total acetate. The pKa of acetic acid = 4.76

isomerization

The isomerization of a phosphorylated gluscose molecule from 1-p to 6-p form with standard free energy change=-1.74kcal/mole. Find the equilibrium concentration ratio of glucose-p in the two isometric states.

Membrane transport

I need a lot of detail on this. Please do not take this unless you can answer it fully. Thank you! Discuss various aspects of membrane transport: passive, facilitated types and active. This needs to be very detailed so I can get a full understanding of it. Thank you

Carbonic Anhydrase - Calculate Turnover Number

Carbonic anhydrase of red blood cells, which has a molecular weight of 30,000, is among the most active known enzymes. It catalyzes the reversible hydration of CO2, which is important for the transport of carbon dioxide from the tissues to the lungs. CO2 + H2O <--> H2CO3 If 10μg of pure carbonic anhydrase catalyzes the hydration of 0.30g CO2 in one minute at 37°C under optimal conditions, calculate the turnover number of carbonic anhydrase.