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Biochemistry, All Topics
Year 3
Carbonic Anhydrase - Calculate Turnover Number
Carbonic anhydrase of red blood cells, which has a molecular weight of 30,000, is among the most active known enzymes. It catalyzes the reversible hydration of CO2, which is important for the transport of carbon dioxide from the tissues to the lungs.
CO2 + H2O <--> H2CO3
If 10μg of pure carbonic anhydrase catalyzes the hydration of 0.30g CO2 in one minute at 37°C under optimal conditions, calculate the turnover number of carbonic anhydrase.
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Enzyme-catalysed reaction: activation energy - Here is the problem I have to solve:
If 10 μg of pure carbonic anhydrase catalyzes the hydration of 0.30 g of carbon dioxide in 1 min at 37˚C under optimal conditions, what is the turnover number (kcat) of carbonic anhydrase (in units of min-1)?
I have already calculated the turnover number, which is 2.0 x 107 min-1, but I'm kind of lo...
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Question:
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