Determining the reaction direction, initial velocity of reaction and the Haldane relationship in enzyme kinetics.
A reversible reaction described by the chemical equation shown below (equation one) has an equilibrium constant of 10. (S represents substrate, P represents product.) If we have a mixture containing [S]=2x10E-5 M and [P]=3x10E-5M, and we know that KmS=3x10E-5 M, Vmax (forward reaction) =2 micromole /(L min), and Vmax (reverse reaction) = 4 micromole /(L min), answer the following questions.
(a) In which direction will the reaction proceed when the appropriate enzyme is added?
(b) After addition of enzyme, what will the initial velocity be when the reaction starts toward equilibrium?
Equation one:
(refer to the attached file for the equation)
By OTA: Monica Tory, MSc
OTA Rating: 3.8/5
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