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Biochemistry, All Topics
Year 2

Enzyme Kinetics: Understanding the Henri-Michaelis-Menten equation/model.


An enzyme with a Km of 0.36 mM was assayed using substrate concentrations of 3 x 10E-7 M, 9.5 x 10E-5 M, 1.5 x 10E-4, 1.16x10E-3, 3 x 10E-3 M, 0.01 M, 0.08 M.  At a substrate concentration of 0.08 M, the initial velocity was 192 nM/min.  Calculate the initial velocity at each of the other substrate concentrations.  Graph the calculated velocities using the Henri-Michaelis-Menten model.

By OTA:  Monica Tory, MSc

OTA Rating:  3.8/5

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    • initial velocity.doc
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